Peridinin-Chlorophyll Protein (PerCP)
Peridinin-Chlorophyll Protein (PerCP) is a red-emitting fluorescent protein originating from dinoflagellates, which are a class of brown microalgae recognized for their distinctive light
The PerCP complex has a high ratio of peridinin to chlorophyll; most light-harvesting complexes contain more chlorophyll than carotenoids, but the PerCP contains eight peridinin and two chlorophyll molecules arranged to promote peridinin-chlorophyll energy transfer. The PerCP complex is a dimer with a molecular weight of approximately 35kDa.
As the leading commercial form of PCPs, PerCP fluorescent labeling is widely applied in medical research . The commercial PerCP complex has a molecular weight of 35 kDa, a broad excitation spectrum (maximum adsorption: 472–488 nm), and a maximum emission around 676 nm . The absorption and emission spectrum are shown in Fig. 2 c.
PerCP is commonly used in research applications such as fluorescence-activated cell sorting (FACS) and flow cytometry. The fluorophore is covalently linked to proteins or antibodies for use in these techniques. Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Jackson ImmunoResearch offers the form found in Dinophyceae sp. It has a broad spectrum of excitation with the main peak at 482 nm, and a long Stokes shift to an emission peak at 677 nm. PerCP conjugates are large complexes suitable for cell surface labeling techniques such as flow cytometry.
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